Proton induced gamma emission (PIG E) analytical technique was used to measure the concentration ofF in groundwater samples collected from different areas of Kalia thana under Narail district. Proton beam of energy 2.5 MeV obtained from the 3 MV Van de Graaff accelerator at the AECD (Atomic Energy Centre, Dhaka) was used for sample irradiation. 200 ml of water sample mixed with 1.0 gm of cellulose powder is evaporated, and the residue is made into standard pellets that are used as target for the proton beam. The characteristic gamma emitted from the exited nuclei of the irradiated samples was detected by a HPGe spectrometer system. Concentrations were obtained by comparing the PIGE gamma yields with that of the standards prepared in the same laboratory. The concentration ranged from 0.71-4.26 mg/L with the mean of 1.73 mg/L. The highest concentration ofF is found at East Durgapur which is 4.26 mg/L and much above the WHO recommended maximum of 1.5 mg/L. The results of the experiments were discussed in the light of elemental deficiencies or the toxicities in the groundwater and the likely impact they may have on the health of the population at that area.