In vitro inhibitory effect of methanol, hexane and ethylacetate extracts of albizia lebbeck (siris tree) stem bark on the activity of alpha glucosidase
Alpha glucosidaseis a potential target enzyme in the management of diabetes mellitus. Diabetes mellitus (DM) is a metabolic disorder caused by defect in insulin secretion, insulin action, or both. Insulin deficiency as such results to a chronic hyperglycemia with disturbances of carbohydrate, fat and protein metabolism. The current work studied the in vitroinhibitory potentials of stem bark extracts of Albizia lebbeck on yeast α-glucosidase activity using p-nitrophenyl-α-D-glucopyronaside (PNPG) as substrate.The Stem Bark of Albizia lebbeckwas collected from the university premises of Usmanu Danfodiyo University Sokoto Nigeria. The stem bark was extracted with methanol and further with hexane and ethyl acetate fractions using separation funnel. Crude α-glucosidase was obtained from yeast (Saccharomyces cereviceae).The hexane and methanol extracts were tested for inhibitory potentials on α-glucosidase and the IC50was calculated. The results indicated that at 10, 30 and 50mg/ml, the hexane extract caused 20.65, 36.52 and 61.96% reduction in the activity of α-glucosidase respectively. The methanol extract on the other hand inhibited the activity of the enzyme by 14.36, 24.30 and 31.29% respectively. The IC50was calculated for the hexane and methanol extracts tobe 1.68 and 2.92mg/ml respectively. The results of this study therefore concluded that hexane and methanol extracts of Albizia lebbeck stem bark possesses significant inhibitory effect on α-glucosidase in a dose dependant manner and may therefore have potentials as a source of lead for the development of anti-diabetic drugs.
In vitro, Inhibitory effect, Albizia lebbeck, Alpha glucosidase
Sirajo Ismail Isah, Bilal SirajoMusa, Nahian Fyrose Fahim, Faisal Muhammad