Ubiquitous nature of erectile dysfunction (ED) has placed it as one of the most rampant health care problem and therefore consumption of energy stimulating herbal medicines (ESHMs) has increased in Bangladesh. However, these herbal medicines reaching consumers without maintaining proper screening procedure, which bring a threat to public health safety. An analysis of biological contaminants (microbial load) of these herbal medicines available in Bangladesh was investigated. In most of samples, the total bacterial counts (TBC) 6 × 10⁷ − 32 × 10¹¹ cfu/mL and lactobacillus count 6 × 10⁸ − 12 × 10¹¹ cfu/mL exceed the maximum value as percribed by WHO.