Tag Archives: Sharifa Sultana

OPTIMIZED AND VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF OLANZAPINE IN PHARMACEUTICAL FORMULATIONS

Author
Sharifa Sultana
Allied Health Science
Daffodil International University

ABSTRACT

A simple, precise, accurate, rapid and reproducible RP-HPLC method has been developed for the determination of Olanzapine in pharmaceutical formulations. Chromatography was carried out on a reverse phase C-8 column (150 x 4.6 mm x 5 µm length), optimum separation was achieved in 15 min using a mobile phase at a flow rate of 1 mL/min and the detection was done at 260 nm. The method produced linear responses in the concentration range from 45.2-135.6 µg/mL of Olanzapine with correlation coefficients of 1, accuracy of 99.80% and precision of 1.885%. The method was found to be reproducible for analysis of the drug in pharmaceutical formulations. The results of the analysis were tested and validated statistically for various parameters according to ICH guidelines and recovery studies confirmed the accuracy of the proposed method.

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RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR NITAZOXANIDE IN POWDER FOR SUSPENSION DOSAGE FORM

Author
Sharifa Sultana
Lecturer
Pharmacy
Daffodil International University
Tanbir Ahammad
Md. Ashraful Islam
Incepta Pharmaceuticals Ltd.

Abstract: 

A simple, precise, accurate, rapid and reproducible RP-HPLC method has been developed for the determination of Nitazoxanide in powder for suspension dosage form. Chromatography was carried out on an ODS C18 column (250 x 4.6 mm x 5 µm length), using a mixture of acetonitrile and 0.005 mol L-1 tetra n- butyl ammonium hydrogen sulphate (54:46 v/v) as the mobile phase at a flow rate of 1 mL/min and the detection was done at 240 nm. The method produced linear responses in the concentration range from 50 to 150 µg/mL of Nitazoxanide with correlation coefficients of 0.999, accuracy of 98.90% and precision of 0.811%. The method was found to be reproducible for analysis of the drug in suspension dosage form. The results of the analysis were tested and validated statistically for various parameters according to ICH guidelines and recovery studies confirmed the accuracy of the proposed method.

For details please see the attached file:

DEVELOPMENT AND IN VITRO EVALUATION OF ONCE DAILY CARBAMAZEPINE MATRIX TABLET FROM HYDROPHILIC POLYMERS

Author
Sharifa Sultana
Pharmacy
Daffodil International University
Harun-Or-Rashid
Pharmacy
University of Dhaka
Shimul Halder
Pharmacy
University of Dhaka

ABSTRACT: 

The purpose of study was to develop and characterize once daily extended release matrix tablets of Carbamazepine (CBZ), an antiepileptic drug. Tablets were prepared by wet granulation method. Methocel K15M CR and Methocel K100LV CR polymers were used as rate retarding agents in fourteen formulations. The granules were evaluated for angle of repose, loose bulk density, tapped bulk density, compressibility index, total porosity, drug content etc. and showed satisfactory results. The tablets were subjected to thickness, weight variation test, drug content, hardness, friability and in vitro release studies. All the tablet formulations showed acceptable pharmacotechnical properties and complied with pharmacopoeial specifications for tested parameters. The in vitro dissolution study was carried out for 24 hour in distilled water as the dissolution medium. The release mechanisms were explored and explained by Zero order, Higuchi, First order, Korsmeyer-Peppas and Hixson-Crowell equations. Primarily nine formulations were prepared by using three variable ratios of the two polymers, with 1% sodium lauryl sulphate. The optimized formulations F-5, F-6 and F-9 were further studied to know the effect of solubilizer on release by using various concentration of sodium lauryl sulphate and glyceryl mono stearate. Kinetic modeling of in vitro dissolution profiles revealed the drug release mechanism followed anomalous type or non-Fickian transport and super case II transport. The release of drug was extended for 24 hour by polymer combinations which indicated the usefulness of the formulations for once daily dosage form. Besides, these studies explored both of the optimum concentration, effect of polymers and the use of sodium lauryl sulphate on CBZ release pattern from the tablet matrix for 24 hour period.
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