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Title
In situ synthesis of monolithic sol–gel polyethylene glycol-based sorbent encapsulated in porous polypropylene microextraction capsules and its application for selective extraction of antifungal and anthelmintic drugs from human urine
Author
, Abuzar Kabir,
Email
Abstract

Herein, a monolithic sol–gel polyethylene glycol-based sorbent (PEG) has been in situ synthesized within two conjoined porous polypropylene microextraction capsules and subsequently applied for the microextraction of triazoles and benzimidazoles drugs from human urine. A cylindrical bar magnet was incorporated into the device, offering the ability of spinning when it is placed in a magnetic field resulting in a stand-alone “all-in-one” extraction device. Among other sorbent tested, the sol–gel PEG 300 coated microextraction capsules were found to be beneficial for the extraction of the target analytes. The analysis of the drugs was performed using high-performance liquid chromatography and photodiode array detection (HPLC-PDA). Detailed investigation of the main parameters that affect the extraction performance (i.e., dimensions of the microextraction capsules, sample volume, extraction time, ionic strength, stirring rate, elution time, type of eluent, volume of eluent) was conducted to find the optimum conditions for the extraction procedure. The method was thoroughly validated using the total-error concept. The β-expectation tolerance intervals did not exceed the acceptance criteria of ± 15% meaning that 95% of future results will be included in the defined bias limits. The lower limits of quantitation (LLOQs) of the method were adequate, corresponding to 0.1 and 0.25 μg mL−1 for triazoles and benzimidazoles, respectively. The mean analytical bias in the spiking levels was satisfactory, ranging between ─ 3.8 to + 6.7 % while the relative standard deviation (RSD) was lower than 5.6 %. Finally, the sol–gel PEG 300 encapsulated microextraction capsules were found to be reusable for at least 15 extraction cycles. The developed approach was successfully applied in the analysis of the selected drugs in urine samples.

Keywords
Capsule phase microextraction Antifungals Anthelmintics Benzimidazoles Urine HPLC-PDA
Journal or Conference Name
Microchemical Journal
Publication Year
2022
Indexing
scopus