This article describes the design of a new Slotted Elliptical Patch with Defected Ground Antenna (SEPDGA) for microwavebased imaging applications. The intended antenna comprises of a defective ground plane and multiple rectangular slotted ellipse-shaped patch. Multi-resonances can be exhibited by the designed antenna which merges to disclose the wideband spectrum. The experimentally verified antenna has a fractional bandwidth (FBW) of 79% and a functional frequency band of 1.3–3.0 GHz (< -10 dB). According to simulation-based outcomes, it operates between 1.4 -3.2 GHz and has an FBW of 78%. Additionally, the antenna prototype has achieved a highest gain of 4.9 dBi at 2.65 GHz and an efficiency peak of above 85%. In addition to demonstrating superior time domain behaviors as well as omnidirectional radiation properties across the entire operating spectrum. Featuring a compact size, decent gain, high fidelity, large band of operation and efficiency, as well as stable omnidirectional radiation characteristics, this antenna is perfect for applications in biomedical imaging.