In this study, green coloured Cu(II) and red coloured Fe(III) complexes of pyridine were prepared and the elemental characterization confirmed their composition. The pyridine complexes of Cu(II) and Fe(III) were found very interesting and attractive as potential candidates with antimicrobial activity. Along with this, melting point, molar conductivity measurement, magnetic moment determination, electronic and FTIR spectroscopy were also measured to characterize the prepared complexes. Pyridine acts as monodentate which resulted in formation of square planar and octahedral structure of Cu(II) and Fe(III) complexes, respectively with the nitrogen atom pyridine ring by coordination bond. For antimicrobial activity studies Alternaria alternata, Bacillus cerelus, Botrgodiplodia theobromal, Colletotrichums corcolei, Escherichia coli, Fusarium equiseti, Macrophomina phaseolina, Salmonell typhi and Shigella dysenteriae were used. The prepared complexes showed inhibition against mycelial growth. Although the complexes showed satisfactory inhibition against the tested bacteria and fungi species, both of the complexes were comparatively better against bacteria than fungi compared to ligand.