A multi band negative index metamaterial based on split square shape resonators has been discussed in this paper. The proposed metamaterial unit cell structure is a combination of split square shape ring resonators. Commercially available electromagnetic simulator CST Microwave Studio has been used to investigate the calculation of the proposed design. The proposed structure shows resonance at 3.36 GHz, 5.98 GHz, 9.83 GHz, 13.10 GHz and exhibits negative index characteristics at 8.25 GHz. The effective medium ratio of the designed unit cell is 8.90. However, a performances comparison analysis has been done between Rogers RT 5880 and Teflon substrate materials.