The Chinese waterchestnut (Eleocharis dulcis) is the corm of a sedge that grows in water and is commonly used in Asian foods. The edible part consists of starch-rich, non-lignified storage parenchyma interspersed with vascular strands. The present study was carried out to investigate phytochemical, antioxidant, antimicrobial, thrombolytic activity and estimation of total flavonoid, total phenolic, total tannin content of Eleocharis dulcis (Cyperaceae) fruits of methanol extract. Total flavonoids content was spectrophotometrically determined using aluminum chloride colorimetric assay while total phenolic and tannin content by Folin Chiocalteu’s reagent. Antioxidant activity was determined by DPPH free radical scavenging. Antimicrobial activity was evaluated by disc diffusion assay. The fruit extract contains greater amount of total flavonoid (79.99 mg QE/g dry extract), phenolic content (35.27 mg GAE/g dry extract) and phenolic content (35.27 mg GAE/g dry extract). Fruit extracts showed DPPH scavenging (157.45 μg/mL) that was comparable to standard ascorbic acid (14.15 μg/mL). In disc diffusion assay extract showed greater antibacterial activity. In the thrombolytic activity extracts showed clot lysis for 200 and 100 mg (86.87%) and (84.15%); respectively. It can be revealed that the fruit extract of Eleocharis dulcis (Cyperaceae) possess antioxidant and antimicrobial and thrombolytic activity. The potential of these activities may be due to the presence of most of the phytochemicals which supports previous claims and validate its uses as an expected folk medicine.