Diabetes is emerging new threats through different complications with time. Present study was designed to evaluate a hypoglycemic activity of Polyalthia longifolia leaves ethanol extract of along with analgesic activity. Oral glucose tolerance test showed significant result at dose 500mg/kg body weight of mice but not significant at dose 250mg/kg compare to control group where metformin was used as a reference standard. Analgesic activity of Polyalthia longifolia extract by acetic acid induced writhing method also showed mentionable effects that was 58% at dose 500mg/kg and 70% at 250mg/kg compare with standard diclofenac 78% writhing inhibition.