Newborn screening (NBS) plays a crucial role in identifying and managing congenital disorders, ultimately leading to improved infant health outcomes. This study aims to assess the current practices of newborn screening in Bangladesh, focusing on the perspectives of healthcare professionals, and identifying potential implications for enhancing infant health. A quantitative research design was employed, utilizing in-depth interviews and focus group discussions with healthcare professionals involved in newborn care across various healthcare settings. Findings indicate that newborn screening practices in Bangladesh face several challenges, including limited awareness among healthcare professionals, inadequate infrastructure and resources, and the absence of a comprehensive national screening program. Furthermore, financial constraints, cultural beliefs, and social barriers contribute to the low utilization of screening services by families. The perspectives of healthcare professionals shed light on potential strategies to address these challenges and improve newborn screening practices in Bangladesh. These strategies include enhancing healthcare professionals' knowledge and skills through training programs, increasing public awareness about the importance of newborn screening, strengthening healthcare infrastructure and accessibility, and implementing a comprehensive national screening program. The implications of improving newborn screening practices are significant. Early detection of congenital disorders through NBS enables timely interventions, leading to reduced morbidity, mortality, and long-term disabilities in infants. Enhanced screening practices can also contribute to reducing the burden on healthcare systems by preventing complications associated with undiagnosed conditions and enabling more cost-effective management. This study highlights the need for comprehensive efforts to address the challenges faced in newborn screening practices in Bangladesh. By incorporating the perspectives of healthcare professionals, policymakers can develop targeted interventions to enhance newborn screening programs, thereby improving infant health outcomes and ensuring a healthier future for Bangladesh's newborn population.