rDNA ITS region Pomegranate (Punica granatum) is cultivated in Bangladesh but domestic production is supplemented by imports from China, India and South Africa. Common postharvest pathogens of pomegranate fruit include Alternaria alternata, Aspergillus niger, Botrytis cinerea and Penicillium spp. (Munhuweyi et al., 2016). In March 2021, imported pomegranate fruit with post-harvest rots were collected from a local market in Gazipur, Bangladesh (23 • 59′ 20.4504'' N, 90 • 25′ 5.4012'' E). Water-soaked spots initially developed and later, dark brown fungal spores were observed on the surface of the fruits (Figure 1). The disease incidence was approximately 15% of the consignment in the market store. Epidermal tissues of diseased fruit were plated on potato dextrose agar medium at 25 • C. The fungus grew rapidly and produced brown to black colonies after three days' incubation (Figure 2a). The conidial head was radiated, vesicles (n = 25) were globose, mean 25.78-50.40 µm, and covered with uniseriate phialides. The stipe of the conidio-phore (n = 25) was hyaline to pale with a mean width and length of 12.14-88.2 µm. Conidia (n = 25) were dark brown, transparent and ellipsoidal with a mean diameter of 3.1-5.54 µm (Figure 2b) (Rahman & Zakaria, 2020). To further ascertain the identity of the isolate, total genomic DNA was extracted using a Wizard DNA purification kit (Promega Corporation , USA) following the manufacturer's protocol. The ITS rDNA gene region was amplified using the primer pair ITS1/ITS4 (White et al., 1990), and the amplified PCR product was sequenced (GenBank This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.