Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis in animals. The present study was conducted to investigate the percentage of toxoplasmosis in goats and their female possessors in the Kurram district Pakistan. A total of 200 blood samples (100 each from goat and their female possessors) were collected randomly from three different regions Malana, Malikhel, and Parachinar of Kurram Pakistan, and were screened for Toxoplasma gondii infection through latex agglutination test and ELISA. Out of 100 goats, 52 were seropositive for toxoplasmosis showing an overall percentage of 52%. The percentage of Toxoplasma gondii was higher in female goats (53.75%) as compared to male goats (45%), and the highest percentage (57.14%) was observed in goats aged more than 2 years. The overall percentage of human toxoplasmosis was 70%. Out of a total of 100 serum samples of female possessors tested 60% were positive for IgM, 10% were positive for IgG and 30 were negative for both IgG and IgM as assessed through ELISA. The high rate of infection in females was observed in age group 46-55 years. The results of the current study reveal that infected goats might be a potential risk for toxoplasmosis in female possessors of Kurram, Khyber Pakhtunkhwa, Pakistan.