The plant-specific TF family ‘Dof’ comprises a highly conserved ‘Dof domain’, which is the DNA binding domain with one ‘Zn2+’ finger. It plays a crucial role in plant responses to environmental stimuli affecting growth and development. The Dof genes have been analyzed in many plants, including Arabidopsis, but have not yet been well studied in sesame on a genome-wide scale. In the present study, we reported for the first time 32 Dof genes in sesame, which are located across 12 linkage groups (LGs). The SiDof genes comprised one to five exons and zero to four introns, which is comparable to Dof genes in other species. Of 32 SiDof genes, about 71.88 % were intronless. The SiDof genes were classified into six groups and exhibited evolutionary relationships with other species. Group III contained the highest number of SiDof genes (seven), while the lowest number was in group V (three). There was a robust protein-protein interaction between NAC071 and two Dof proteins, namely DOF5.3 and DOF5.6. Total 1668 ‘cis-regulatory elements’ were identified in the promoter of 32 SiDof genes. Among these, 733 were linked to the stress responses, 562 to the light responses, and 373 to the phytohormone response. The accessible transcriptome (‘RNA-seq’) data-based expression profiles have shown that SiDof genes might be associated with various abiotic stresses, including drought (SiDof1) and salinity (SiDof32) in sesame. Nonetheless, further investigation on the functionality analysis of candidate SiDof genes is required.