Early diagnosis and intervention are crucial for improving clinical outcomes in patients with cystic fibrosis (CF), a life-shortening genetic disorder, making the development of cost-effective and reliable analytical systems for detecting the mutant gene essential. In this research, an innovative approach leveraging the synergistic use of gold nanorods (GNRs) as catalysts and fabricated aluminium interdigitated tautochrone-curve electrodes (ITE) is proposed for recognising CF-associated mutated deoxyribonucleic acid sequence (m-DNA) in small volumes. The concentration of m-DNA was determined by evaluating variations in the impedance of the electrochemical system, with the real and imaginary components serving as variables. Although both types of impedance variables exhibited excellent linear correlations with the concentration, the DNA analysis relying on charge transfer resistance (Rct) achieved ∼86 % higher sensitivity than the one based on maximum imaginary impedance (Z"max). Limit of detection (LOD) for Rct was lower than that for Z"max, with values of 2.18 and 5.49 fM, respectively. Specificity analysis was additionally conducted using different concentrations of non-complementary wild-type DNA sequence (wt-DNA), and the results demonstrated that the Rct-based approach was reliable when the LOD was used as a threshold. This study highlights the potential of GNR-integrated aluminium ITE (GNR-ITE) for quantifying DNA macromolecules and provides insights into interfacial electrochemical reactions.