The Rema Kalenga is the second-largest natural forest in Bangladesh, after the Sundarbans. A safe haven for wildlife, this dry woodland has been designated a “Reserve Forest” by the government of Bangladesh. The study is focused on the effects of anthropogenic activities on the biodiversity as well as the social impacts in the Rema-Kalenga Forest area. The impact of Covid-19 on the biodiversity and local communities also reflected in the study. The study is conducted using remote-sensing tools and survey methods including questionnaire, focus group discussion and field survey. The decrease of forest area and increase of commercial agricultural land is identified from 1994 to 2022 by Landsat image. Anthropogenic activities like illegal tree cutting, vegetation, tourism have significant impact on the biodiversity of the forest area. However, the majority of respondents claim that they preserve the forest against illegal activities and, if necessary, will plant more trees. The Covid-19 reduced human footprint around the forest area, which help to improve the biodiversity of the area. On the other hand, local communities lost their income source due to the absence of tourists, which further forced them to involve in illegal activities. However, the Covid-19 teach the communities a valuable lesson about the dependency of the forest and the nature.