Isoprene is one of the most abundant biogenic volatile organic compounds (BVOCs) emitted by plants and plays a key role in atmospheric chemistry. However, seasonally resolved, species-specific outdoor enclosure-based sapling measurements in tropical ecosystems remain scarce. In this study, isoprene emission rates from sixteen dominant tropical tree species in Central India were characterized across summer, rainy, and winter seasons using a dynamic enclosure chamber with potted saplings at GGV Campus, Bilaspur. Isoprene emissions peaked in summer, contributing 52% of total annual emissions, followed by rainy (27%) and winter (21%) seasons. Among the species studied, Eucalyptus globulus exhibited the highest standard emission rate during summer. Emissions showed strong positive correlations with temperature (TEM) and photosynthetically active radiation (PAR), while correlations with relative humidity (RH) and carbon dioxide (CO₂) were generally weak. The observed seasonal variability reflects the combined influence of meteorological drivers and species-specific emission capacities. This study provides regionally relevant standard emission factors (Eₛ) for key tropical species in Central India, which can support improved modeling of BVOC emissions and regional atmospheric chemistry. These results represent controlled outdoor sapling measurements and should not be interpreted as mature forest canopy fluxes; the absolute emission values should therefore be treated as method-constrained estimates.