Gynura nepalensisleaves have been reported to use traditionally in the treatment of diabetes as well as in indigestion and healing of wounds. The present study investigated the antihyperlipidemic potential of the leaf extract from Gynura nepalensis(GN). Experimental hyperlipidemia was induced by oral administration of animal fat for 30 days. After 30 days treatment with fat, hyperlipidemic rats were divided into different groups (n=6) and administered test extract along with animal fat for further 28 days. After 28 days treatment, rats were sacrificed, blood samples were collected; serum was separated and subjected to lipid profile test such as TC, TG, VLDL-C, LDL-C and HDL-C.Administration of extra animal fat along with normal diet in rats produced hyperlipidemia. After28 days treatment of hyperlipidemic rats with GN leaves extract, GN 250mg/kg showed significant reduction of TC (p<0.01), TG (p<0.05) and LDL-C (p<0.01) level whereas GN 500mg/kg dose demonstrated significant reduction of TC (p<0.001), TG (p<0.001) and LDL-C (p<0.001) level. Also insignificantly reduced VLDL-C and insignificantly increased the HDL-C at both the doses as compared to the lipid control group.Based on this investigation, G. nepalensisleaves exhibited antihyperlipidemic effect by amelioratinglipid metabolism disorders in hyperlipidemic model rats. However, the specific mechanism (s) by which GN induces its effects on hyperlipidemia needs further investigation.